Isolation, Characterization and Identification of Bacteria from Homemade Fertilizer
DOI:
https://doi.org/10.11113/jomalisc.v2.35Keywords:
Homemade fertilizer, purple non-sulfur bacteria, Clostridium spp., indole acetic acid (IAA), aminolevulinic acid (ALA)Abstract
The rising demand for natural resources has resulted in the usage of chemical fertilizers. Biological fertilizers must be mandated to safeguard humans and the environment from toxic chemical fertilizers. As a result, this study focuses on isolating, characterizing, and identifying bacteria from homemade biofertilizers, which are environmentally friendly and help boost plant growth. Two distinct media of purple non-sulfur bacteria enrichment medium (PNSBEM) and nutrient agar were used to grow bacteria isolated from the homemade fertilizer. The optimum bacterial growth study was conducted under aerobic, facultative, and obligate anaerobic conditions. Further characterization of isolated bacteria, including Gram staining, catalase assay, pathogenicity assay and partial 16s rRNA sequencing, reflects Gram-positive Clostridium sp. The bacteria isolated from the homemade fertilizer showed its ability to synthesize auxin components such as Indole acetic acid (IAA) and aminolevulinic acid (ALA). Homemade fertilizer has the potential to be an effective biofertilizer since it shows a favourable outcome in the IAA component, which is vital in controlling plant growth and development.
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