Magnetic-Solid Phase Extraction of 5-Fluorouracil Using Newly Synthesized Surface-Imprinted Magnetic Hollow Porous Polymer
DOI:
https://doi.org/10.11113/jomalisc.v3.49Keywords:
Molecularly imprinted polymer, magnetic-solid phase extraction, 5-fluorouracil, capillary electrophoresisAbstract
A magnetic hollow porous molecularly imprinted polymer (MHPMIP) was developed using a surface imprinting technique (mesoporous MCM-48 as a core). Its potential as a magnetic-solid phase extraction (MSPE) sorbent for the extraction of 5-fluorouracil (5-FU) anticancer drug was investigated to overcome some drawbacks caused by conventional SPE sorbents. Although SPE offers advantages such as rapidity and simplicity, the issues of low selectivity and sensitivity still remain unsolved. Moreover, the current determination methods of 5-FU in water samples are seriously disadvantaged due to major matrix interferences. Thus, extensive sample preparation is required before instrumental analysis. The MHPMIP was synthesized via precipitation polymerization using a functional monomer, methacrylic acid (MAA), crosslinking agent, ethylene glycol dimethacrylate (EGDMA), an initiator 4,4’-azobis (4-cyanopentanoic acid) (ACPA), and also the template, 5-FU. The specific surface area of MHPMIP was determined to be 63.47 m2/g with a pore volume of 0.2325 cm3/g using Brunauer-Emmett-Teller (BET) method. The MHPMIP possessed a good adsorption capacity of 2.089 mg/g. The adsorption capacity of 5-FU was significantly higher than that of the non-imprinted one (MHPNIP) (0.02324 mg/g). Coupled with FASI-CZE, the MSPE using the MHPMIP as sorbent showed that it could extract 5-FU from wastewater samples at low ppm levels. The limit of detection (LOD) and the limit of quantitation (LOQ) of the MSPE-FASI-CZE method was found to be 3.228 and 9.782 mg/L, respectively, in the linear concentration range of 5 to 15 mg/L with a good correlation (R2 = 0.9973). A recovery up to 105.7% (RSD% 9.428, n = 3) was obtained for 5-FU (5 mg/L) spiked in real samples.
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