An Elaeocarpus grandiflorus Leaves Extract Increased Immunoglobulin G Activity and Total Protein of SRBC-Induced Rat
DOI:
https://doi.org/10.11113/jomalisc.v3.66Keywords:
Elaeocarpus grandiflorus, immunoglobulin G, immunostimulantAbstract
The immune system plays a critical role in defending against viral infections such as Covid-19. Elaeocarpus grandiflorus contains various active compounds, including flavonoids, saponins, polyphenols, and tannins. Among these, the primary flavonoids identified in E. grandiflorus are kaempferol, quercetin, procyanidin, naringin, orientin, iso-orientin, vitexin, isovitexin, rutin, luteolin, and epicatechin. Kaempferol and quercetin have been particularly noted for their anti-inflammatory properties and their potential to enhance the immune system. This study aimed to investigate the effects of E. grandiflorus leaf extract on immunoglobulin G (IgG) activity and total protein levels in rats induced by sheep red blood cells (SRBC). Twenty-five Wistar rats were randomly assigned to five groups: P1, P2, P3, Negative control, and Positive control. The extract suspension was administered from day 1 to day 7 at concentrations of 100 mg/kg BW (P1), 200 mg/kg BW (P2), and 400 mg/kg BW (P3). On day 7, rats were intraperitoneally injected with 1 mL of a 2% v/v solution of SRBC as an antigen. IgG activity and total protein levels were assessed on day 12. The average agglutination titters were as follows: 3.65 (Negative control), 3.89 (Positive control), 3.77 (P1), 3.65 (P2), and 4.61 (P3). Additionally, the average total protein values for the groups were 1.44 mg/dL (Positive control), 1.00 mg/dL (Negative control), 1.18 mg/dL (P1), 1.22 mg/dL (P2), and 1.46 mg/dL (P3). Significant differences were observed in IgG activity and total serum protein levels among the groups. These findings suggest a potential immunostimulant effect of E. grandiflorus leaf extract and warrant further exploration for its development.
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