Alginate Chitosan Amine-Functionalized Silica-Coated as an Adsorbent for Magnetic Micro Solid-Phase Extraction of Pyrocatechol in Coffee Sample
Keywords:
magnetic micro-solid phase extraction, pyrocatechol, coffee analysis, biopolymer functionalized adsorbent, UV-Vis spectrophotometryAbstract
This study aimed to optimize the parameters for magnetic solid-phase extraction (MSPE) for the analysis of pyrocatechol in coffee using ultraviolet-visible (UV-Vis) spectrophotometry instrument. The magnetic solid phase extraction (MSPE) adsorbent used was alginate chitosan amine-functionalized silica-coated (alg/Cs-NH2-SiO2/Fe3O4). The effect of desorption solvent, elution time, and mass of sorbent were investigated. The calibration curve showed a straight line with R2 = 0.9985, which showed that there was a linear relationship between the concentration of the substance and its response (absorbance). As the curve was linear, it showed that the instrument’s response was directly proportional to the concentration within the measured range. The optimum MSPE conditions were ethanol as the desorption solvent, 80 mg of alg/Cs-NH2-SiO2/Fe3O4 adsorbent and 30 seconds of extraction time. Under the optimized conditions, the MSPE method showed good linearity in the range of 1-10 mg/L with coefficient of determination R2 = 0.9965. The method showed limit of detection (LOD) and limit of quantification that were calculated to be 1.0 mg/L. Good precision achieved with relative standard deviations (RSD) < 10% and good repeatability 0.55%. The developed method was successfully applied to the analysis of pyrocatechol in coffee. The consistency of absorbance values observed across multiple measurements (1.438, 1.430, and 1.435) suggests reproducibility and reliability of the experimental results. These findings demonstrate the stability and robustness of the UV-Vis analysis technique used in this study.
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